bbs1 digested px459 Search Results


bbs1 digested px459  (Addgene inc)
96
Addgene inc bbs1 digested px459
Figure 2. Construction and validation of the CRISPR/Cas9 system. (A) The structure of the ARID4B protein with a total of 1312 amino acids and the site where the mutations were generated in position 939, indicated as a hotspot. (B) Design of the sgRNA and validation of the ligation in the <t>pX459</t> vector by Sanger sequencing. Only underlined region is shown. Red for T, Black for G, Blue for C, and Green for A. (C) Confirmation of the presence of genetic editions in the pool of MCF-7 cells after transfection. The surveyor assay produces a cut in the PCR product, generating two fragments indicated by arrows. (D) Validation of the presence of genetic editions in isolated clones. Here, 3, 10, and 21 represent the clones positive for mutations in ARID4B.
Bbs1 Digested Px459, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bbs1+digested+px459/pSpCas9(BB)-2A-Puro+(PX459)+V2%2E0+(Plasmid+%2362988)/pm38137006-60-7-11
Average 96 stars, based on 1 article reviews
bbs1 digested px459 - by Bioz Stars, 2026-10
96/100 stars
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bbs1 digested pspcas9 bb 2a puro px459v2 plasmid  (Addgene inc)
96
Addgene inc bbs1 digested pspcas9 bb 2a puro px459v2 plasmid
Figure 2. Construction and validation of the CRISPR/Cas9 system. (A) The structure of the ARID4B protein with a total of 1312 amino acids and the site where the mutations were generated in position 939, indicated as a hotspot. (B) Design of the sgRNA and validation of the ligation in the <t>pX459</t> vector by Sanger sequencing. Only underlined region is shown. Red for T, Black for G, Blue for C, and Green for A. (C) Confirmation of the presence of genetic editions in the pool of MCF-7 cells after transfection. The surveyor assay produces a cut in the PCR product, generating two fragments indicated by arrows. (D) Validation of the presence of genetic editions in isolated clones. Here, 3, 10, and 21 represent the clones positive for mutations in ARID4B.
Bbs1 Digested Pspcas9 Bb 2a Puro Px459v2 Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bbs1+digested+px459/pSpCas9(BB)-2A-Puro+(PX459)+(Plasmid+%2348139)/pmc09525119__jci___132___162365___s008-12-19-29
Average 96 stars, based on 1 article reviews
bbs1 digested pspcas9 bb 2a puro px459v2 plasmid - by Bioz Stars, 2026-10
96/100 stars
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spcas9  (Addgene inc)
96
Addgene inc spcas9
Figure 2. Construction and validation of the CRISPR/Cas9 system. (A) The structure of the ARID4B protein with a total of 1312 amino acids and the site where the mutations were generated in position 939, indicated as a hotspot. (B) Design of the sgRNA and validation of the ligation in the <t>pX459</t> vector by Sanger sequencing. Only underlined region is shown. Red for T, Black for G, Blue for C, and Green for A. (C) Confirmation of the presence of genetic editions in the pool of MCF-7 cells after transfection. The surveyor assay produces a cut in the PCR product, generating two fragments indicated by arrows. (D) Validation of the presence of genetic editions in isolated clones. Here, 3, 10, and 21 represent the clones positive for mutations in ARID4B.
Spcas9, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bbs1+digested+px459/SP-Cas9+(Plasmid+%2362731)/pmc06060633-125-10-14
Average 96 stars, based on 1 article reviews
spcas9 - by Bioz Stars, 2026-10
96/100 stars
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px459 vector  (Addgene inc)
96
Addgene inc px459 vector
Figure 2. Construction and validation of the CRISPR/Cas9 system. (A) The structure of the ARID4B protein with a total of 1312 amino acids and the site where the mutations were generated in position 939, indicated as a hotspot. (B) Design of the sgRNA and validation of the ligation in the <t>pX459</t> vector by Sanger sequencing. Only underlined region is shown. Red for T, Black for G, Blue for C, and Green for A. (C) Confirmation of the presence of genetic editions in the pool of MCF-7 cells after transfection. The surveyor assay produces a cut in the PCR product, generating two fragments indicated by arrows. (D) Validation of the presence of genetic editions in isolated clones. Here, 3, 10, and 21 represent the clones positive for mutations in ARID4B.
Px459 Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bbs1+digested+px459/pcDNA3%2E1(%2B)+Laccase2+MCS+Exon+Vector+(Plasmid+%2369893)/10__1091_slash_mbc__e16___01___0003-195-8-16
Average 96 stars, based on 1 article reviews
px459 vector - by Bioz Stars, 2026-10
96/100 stars
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ecor1 digested plko tet on  (Addgene inc)
96
Addgene inc ecor1 digested plko tet on
Figure 2. Construction and validation of the CRISPR/Cas9 system. (A) The structure of the ARID4B protein with a total of 1312 amino acids and the site where the mutations were generated in position 939, indicated as a hotspot. (B) Design of the sgRNA and validation of the ligation in the <t>pX459</t> vector by Sanger sequencing. Only underlined region is shown. Red for T, Black for G, Blue for C, and Green for A. (C) Confirmation of the presence of genetic editions in the pool of MCF-7 cells after transfection. The surveyor assay produces a cut in the PCR product, generating two fragments indicated by arrows. (D) Validation of the presence of genetic editions in isolated clones. Here, 3, 10, and 21 represent the clones positive for mutations in ARID4B.
Ecor1 Digested Plko Tet On, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bbs1+digested+px459/Tet-pLKO-puro+(Plasmid+%2321915)/pm41120304-242-28-30
Average 96 stars, based on 1 article reviews
ecor1 digested plko tet on - by Bioz Stars, 2026-10
96/100 stars
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single-stranded dna  (Integrated DNA Technologies)
95
Integrated DNA Technologies single-stranded dna
Figure 2. Construction and validation of the CRISPR/Cas9 system. (A) The structure of the ARID4B protein with a total of 1312 amino acids and the site where the mutations were generated in position 939, indicated as a hotspot. (B) Design of the sgRNA and validation of the ligation in the <t>pX459</t> vector by Sanger sequencing. Only underlined region is shown. Red for T, Black for G, Blue for C, and Green for A. (C) Confirmation of the presence of genetic editions in the pool of MCF-7 cells after transfection. The surveyor assay produces a cut in the PCR product, generating two fragments indicated by arrows. (D) Validation of the presence of genetic editions in isolated clones. Here, 3, 10, and 21 represent the clones positive for mutations in ARID4B.
Single Stranded Dna, supplied by Integrated DNA Technologies, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bbs1+digested+px459/Single-stranded+DNA/custom%40single-stranded-dna%4031851326
Average 95 stars, based on 1 article reviews
single-stranded dna - by Bioz Stars, 2026-10
95/100 stars
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lenticrispr v2  (Addgene inc)
96
Addgene inc lenticrispr v2
Figure 2. Construction and validation of the CRISPR/Cas9 system. (A) The structure of the ARID4B protein with a total of 1312 amino acids and the site where the mutations were generated in position 939, indicated as a hotspot. (B) Design of the sgRNA and validation of the ligation in the <t>pX459</t> vector by Sanger sequencing. Only underlined region is shown. Red for T, Black for G, Blue for C, and Green for A. (C) Confirmation of the presence of genetic editions in the pool of MCF-7 cells after transfection. The surveyor assay produces a cut in the PCR product, generating two fragments indicated by arrows. (D) Validation of the presence of genetic editions in isolated clones. Here, 3, 10, and 21 represent the clones positive for mutations in ARID4B.
Lenticrispr V2, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bbs1+digested+px459/lentiCRISPR+v2+(Plasmid+%2352961)/pm41120304-242-14-16
Average 96 stars, based on 1 article reviews
lenticrispr v2 - by Bioz Stars, 2026-10
96/100 stars
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qiaquick gel extraction kit  (Qiagen)
97
Qiagen qiaquick gel extraction kit
Figure 2. Construction and validation of the CRISPR/Cas9 system. (A) The structure of the ARID4B protein with a total of 1312 amino acids and the site where the mutations were generated in position 939, indicated as a hotspot. (B) Design of the sgRNA and validation of the ligation in the <t>pX459</t> vector by Sanger sequencing. Only underlined region is shown. Red for T, Black for G, Blue for C, and Green for A. (C) Confirmation of the presence of genetic editions in the pool of MCF-7 cells after transfection. The surveyor assay produces a cut in the PCR product, generating two fragments indicated by arrows. (D) Validation of the presence of genetic editions in isolated clones. Here, 3, 10, and 21 represent the clones positive for mutations in ARID4B.
Qiaquick Gel Extraction Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bbs1+digested+px459/QIAquick+Gel+Extraction+Kit/ppr0421356-186-20-24
Average 97 stars, based on 1 article reviews
qiaquick gel extraction kit - by Bioz Stars, 2026-10
97/100 stars
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Image Search Results


Figure 2. Construction and validation of the CRISPR/Cas9 system. (A) The structure of the ARID4B protein with a total of 1312 amino acids and the site where the mutations were generated in position 939, indicated as a hotspot. (B) Design of the sgRNA and validation of the ligation in the pX459 vector by Sanger sequencing. Only underlined region is shown. Red for T, Black for G, Blue for C, and Green for A. (C) Confirmation of the presence of genetic editions in the pool of MCF-7 cells after transfection. The surveyor assay produces a cut in the PCR product, generating two fragments indicated by arrows. (D) Validation of the presence of genetic editions in isolated clones. Here, 3, 10, and 21 represent the clones positive for mutations in ARID4B.

Journal: Genes

Article Title: Heterozygous Knockout of ARID4B Using CRISPR/Cas9 Attenuates Some Aggressive Phenotypes in a Breast Cancer Cell Line.

doi: 10.3390/genes14122184

Figure Lengend Snippet: Figure 2. Construction and validation of the CRISPR/Cas9 system. (A) The structure of the ARID4B protein with a total of 1312 amino acids and the site where the mutations were generated in position 939, indicated as a hotspot. (B) Design of the sgRNA and validation of the ligation in the pX459 vector by Sanger sequencing. Only underlined region is shown. Red for T, Black for G, Blue for C, and Green for A. (C) Confirmation of the presence of genetic editions in the pool of MCF-7 cells after transfection. The surveyor assay produces a cut in the PCR product, generating two fragments indicated by arrows. (D) Validation of the presence of genetic editions in isolated clones. Here, 3, 10, and 21 represent the clones positive for mutations in ARID4B.

Article Snippet: Both oligos were aligned and cloned in Bbs1 digested pX459 (62988, Addgene, Cambridge, MA, USA).

Techniques: Biomarker Discovery, CRISPR, Generated, Ligation, Plasmid Preparation, Sequencing, Transfection, Isolation, Clone Assay